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No.
V5R4 and V6R1 System i Access for Windows support the Windows
2008 Server Editions: Standard, Enterprise, Datacenter and Web
Server. V5R4 requires service pack PTF SI25949 or later.
V5R4, V6R1 and later System i Access for Windows do not support
the Windows 2008 Server Core Installation option.
There are no plans to support Windows 2008 Servers with V5R3
System i Access for Windows.