One of the things that sysprep is supposed to do is strip the SID.
To quote from knowledgebase article 298491:
“One problem from duplicating an installation of Windows 2000 is that each cloned computer has the same security identifier (SID) and computer name. This may prevent the cloned computers from functioning correctly in a workgroup or a domain. To work around this problem, administrators use the System Preparation Tool (Sysprep.exe) to remove configuration settings that are unique to the computer such as the computer name and SID. The resulting image can then be safely reused for installation on other computers.”