It will work but realize you have the potential to lose information in between the sync and should your domain controllers go down. If you synced on Friday, shut it down, and then on Thursday night all domain controllers go down, you will lose all changes in between then including new users, password changes, group memberships, new computers, new servers, etc. Just keep this in mind.
But to answer your question, yes it will work. If you wanted to take it a step further, make this new server virtual so you can make a copy of it onto a large enough USB drive or even tape.